Professor of Optical Engineering
Shenzhen University

Super-resolution for live cell imaging

Super-resolution imaging has made remarkable progress in recent years, providing a powerful tool for biology. It allows for the observation of fine structures of cells, cellular dynamics and cellular functions at nanometer scale or even single molecular level, which greatly promotes the development of life science and many other fields. In this talk I will present our recent work in super-resolution optical microscopy. By combining stimulation emission depletion (STED) microscopy and fluorescence lifetime imaging (FLIM), we can improve the spatial resolution of STED and perform FLIM imaging at nanometer resolution. Novel fluorescent probes with low STED laser power and experiment strategies were designed for live cell mitochondria imaging. Coherent adaptive optical technique (COAT) has been implemented in STED microscope to circumvent the scattering and aberration effect for thick sample imaging. Stochastic optical reconstruction microscopy (STORM) superresolution imaging of mitochondrial membrane in live cells was achieved by the development of new fluorescent probes, improved imaging system and optimized single molecule localization algorithm. These developments make it possible to study dynamic events and complex functions in living cells, even with a single conventional microscope.

For more information or to schedule a meeting with the speaker, please contact Hanna Kim.
Hosted by Dr. Zhongping Chen