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Laser Microbeam and Medical Program (LAMMP) Seminar

Rakesh Patalay, MBBS MRCP (UK) PhD
Imperial College NHS Trust, London
Dr Rakesh Patalay graduated from University College Medical School, London in 1999. He completed his general internal medical training before training as a dermatologist in 2003 working in many of the major teaching hospitals in London. He was awarded a Mohs dermatology surgical fellowship at the prestigious St John.s Institute of Dermatology, London between 2012-13 and is a fully certified dermatologist and dermatology surgeon.

He completed a PhD at Imperial College London in Optics in 2012 developing non-invasive fluorescence imaging techniques for the diagnosis of skin cancer, specifically, the development of multispectral fluorescence lifetime imaging. His current research interests include developing non-invasive imaging modalities for skin cancer and skin cancer surgery. He currently works at Imperial College NHS Trust, London.
Do we still need pathologists? Advances in non-invasive imaging of skin using multiphoton microscopy with fluorescence lifetime imaging.
Thursday, May 15, 2014
Beckman Laser Institute and Medical Clinic Library
Noon - 1 PM
(Lunch provided)
Abstract
In recent years there has been a growing clinical interest and availability of non-invasive imaging devices specifically designed to look at the skin. These modalities include reflectance confocal microcopy, optical coherence tomography and optoacoustic imaging.

Of the currently available imaging modalities, the strength of multiphoton fluorescence microscopy (MPM) is that optically sectioned subcellular imaging is possible with a high spatial resolution in a scattering medium such as the skin. Fluorescence lifetime measurements of autofluorescent compounds can be combined with MPM and has been shown to differ between normal and diseased tissue. Therefore, the combination of fluorescence lifetime imaging (FLIM) and MPM is an attractive technique for the diagnosis of skin disease and lesions and the detection of tumour margins.

At Imperial College London, in collaboration with JenLab, we have developed a multispectral FLIM detector and a hyperspectral imager that we have integrated into the commercially available two-photon DermaInspect® tomograph. It has allowed us to explore its potential for the differentiation of normal skin and skin cancers and the difference between normal moles and melanomas. I will be presenting some of this work that demonstrates the current limits of what is achievable with MPM FLIM imaging both in vivo and ex vivo. The data highlights the potential of spectrally resolved FLIM as a realistic diagnostic aid in the Dermatology clinic.